Quantifying Antibiotic Resistance and Antibiotic Resistance Selective Chemicals In The River Almond Catchment Area – A Baseline For Risk Assessment And Intervention Targets
- To determine the prevalence, drivers and sources of AMR pollution in the River Almond.
- Evaluate several phenotypic and genotypic bacterial surveillance markers to determine their suitability in environmental monitoring of AMR.
- Quantify the abundance of antibiotic resistant bacteria (ARB) and antibiotic resistant genes (ARG) at five sites (including sites with high and low anthropogenic pressure) on the River Almond using culture-dependent and culture-independent techniques.
- Quantify the concentration of antibiotic resistance selective chemicals at each site and assess correlation with the abundance of ARB and ARG.
- Determine the point and diffuse sources of contamination of antibiotic resistance in the environment using microbial source tracking and bacterial strain characterisation methods.
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